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The role of the microtubule-severing enzyme katanin in development, memory formation and consolidation

Date: Monday, February 3, 2020 10:00 - 11:00
Speaker: Franco Luis Lombino (ZMNH, Hamburg)
Location: Mondi Seminar Room 3, Central Building
Series: Life Sciences Seminar
Host: Ryuichi Shigemoto
Contact: MARR Lena

Microtubules (MTs) are involved in a variety of cellular functions throughout the entire neuronal
lifespan, such as cell division and intracellular cargo-transport. Katanin is composed by the
regulatory subunit p80 and the catalytic subunit p60. It is an enzyme that forms a hexameric
ring and severs MTs via the hydrolysis of ATP, and although previous data indicate that katanin
plays a role in cell division and brain cortex formation, little is known about the function of this
enzyme during brain development in vivo and in adult neurons.
In our study, we generated a constitutive knockout mouse for Katna1, which encodes the
catalytic subunit of katanin and investigated its role in brain development using a combination
of immunohistochemical, biochemical and in vivo techniques. On top, we made use of an
ATPase-deficient p60 construct (p60-DEID) in order to dissect its role in adult neurons in vitro.
On the one hand, our results indicate that Katna1 homozygous knockout mice do not survive,
in agreement with a role of p60 katanin in cell division. In addition, we observed defects during
embryonic and adult dentate gyrus (DG) neurogenesis in Katna1 heterozygous mice, and
these defects did not impair hippocampal plasticity or learning and memory in behavioral tasks.
On the other hand, our unpublished data suggest that p60 katanin is involved in synaptic
plasticity in adult hippocampal neurons. Altogether, we provide evidence that MT-severing by
katanin plays a major role during development as well as in the adult brain.
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